Beta-actin was detected as the endogenous control for (ISOC1 OE) and miR-4633 stably overexpressed NSCLC cell lines, the full-length of and miR-4633 were amplified from complementary DNA (cDNA) and genomic DNA (gDNA) from the NSCLC cell line A549 by PCR and were cloned into the pCDH-CMV-MCS-EF1-GFP+Puro (CD513B) plasmid (System Biosciences, USA) - PARP Inhibitor expression in the rat pup model

Beta-actin was detected as the endogenous control for (ISOC1 OE) and miR-4633 stably overexpressed NSCLC cell lines, the full-length of and miR-4633 were amplified from complementary DNA (cDNA) and genomic DNA (gDNA) from the NSCLC cell line A549 by PCR and were cloned into the pCDH-CMV-MCS-EF1-GFP+Puro (CD513B) plasmid (System Biosciences, … Continue reading Beta-actin was detected as the endogenous control for (ISOC1 OE) and miR-4633 stably overexpressed NSCLC cell lines, the full-length of and miR-4633 were amplified from complementary DNA (cDNA) and genomic DNA (gDNA) from the NSCLC cell line A549 by PCR and were cloned into the pCDH-CMV-MCS-EF1-GFP+Puro (CD513B) plasmid (System Biosciences, USA)